منابع مشابه
Gating NO release from nitric oxide synthase.
We have investigated the kinetics of NO escape from Geobacillus stearothermophilus nitric oxide synthase (gsNOS). Previous work indicated that NO release was gated at position 223 in mammalian enzymes; our kinetics experiments include mutants at that position along with measurements on the wild type enzyme. Employing stopped-flow UV-vis methods, reactions were triggered by mixing a reduced enzy...
متن کاملNO-synthase inhibition in sepsis.
Thromb Haemost 2006; 95: 591–2 Despite major advances and novel therapeutic approaches in intensive care medicine, sepsis is still a major burden of modern medicine and the leading cause of death in non-coronary intensive care units (1). Sepsis represents a complex disease state with distinctive activation of the inflammation cascade and the coagulation system, resulting in pervasive macroand m...
متن کاملMicrodialysis-based analysis of interstitial NO in situ: NO synthase-independent NO formation during myocardial ischemia.
OBJECTIVES Nitric oxide (NO) synthesis by NO synthases (NOS) requires oxygen. However, although counterintuitive, NO synthesis is increased in ischemic myocardium. Accordingly, mechanisms independent of the NOS pathway have been suggested to contribute to NO synthesis during ischemia. NO initiates detrimental as well as protective mechanisms in a concentration-dependent manner, thus aggravating...
متن کاملNormal vascular development in mice deficient in endothelial NO synthase: possible role of neuronal NO synthase.
PURPOSE Nitric oxide formation by nitric oxide synthase (NOS) has been implicated in vascular injury and retinal neovascularization during oxygen-induced retinopathy. However, the role of NOS in normal retinal vascular development and growth has not been studied. The purpose of these experiments was to characterize the expression of NOS in relation to vascular development and to determine the e...
متن کاملNO synthase inhibition increases aldosterone in humans.
To test the hypothesis that NO influences aldosterone production in humans, we examined the effect of N(G)-nitro-L-arginine methyl ester (L-NAME) on aldosterone concentrations in the presence and absence of the NO precursor L-arginine (3 g TID) and the angiotensin-converting enzyme inhibitor ramipril (10 mg QD). Ten normal subjects were given L-NAME (66 microg/kg per min for 30 minutes) or vehi...
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ژورنال
عنوان ژورنال: Proceedings of the National Academy of Sciences
سال: 1996
ISSN: 0027-8424,1091-6490
DOI: 10.1073/pnas.93.25.14492